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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
06/12/2021 |
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Data da última atualização: |
06/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIMKIĆ, I.; BHARDWAJ, V.; CARPENTIERI-PIPOLO, V.; KUZMANOVIĆ, N.; DEGRASSI, G. |
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Afiliação: |
IVICA DIMKIĆ, Department of Biochemistry and Molecular Biology, University of Belgrade – Faculty of Biology, Belgrade, Serbia; VIBHA BHARDWAJ, Ras Al Khaimah Municipality Department, Director Environment Laboratories, Dubai, United Arab Emirate; VALERIA CARPENTIERI PIPOLO, CNPT; NEMANJA KUZMANOVIĆ, Federal Research Centre for Cultivated Plants (JKI), Institute for Plant Protection in Horticulture and Forests, Julius Ku¨ hn-Institut, Braunschweig, Germany; GIULIANO DEGRASSI, Industrial Biotechnology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina. |
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Título: |
The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plos One, v. 16, n.11, e0259465 Jan. 2021. |
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DOI: |
https://doi.org/10.1371/journal. pone.0259465 |
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Idioma: |
Inglês |
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Conteúdo: |
Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader. MenosCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides.... Mostrar Tudo |
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Palavras-Chave: |
Curtobacterium sp; Enzyme; GD1. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228529/1/journal.pone.0259465-PLOSONE-2021.pdf
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Marc: |
LEADER 02369naa a2200229 a 4500
001 2137133
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal. pone.0259465$2DOI
100 1 $aDIMKIĆ, I.
245 $aThe chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.$h[electronic resource]
260 $c2021
520 $aCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm � 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
650 $aSoybeans
653 $aCurtobacterium sp
653 $aEnzyme
653 $aGD1
700 1 $aBHARDWAJ, V.
700 1 $aCARPENTIERI-PIPOLO, V.
700 1 $aKUZMANOVIĆ, N.
700 1 $aDEGRASSI, G.
773 $tPlos One$gv. 16, n.11, e0259465 Jan. 2021.
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Registro original: |
Embrapa Trigo (CNPT) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
27/01/1995 |
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Data da última atualização: |
10/01/2013 |
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Autoria: |
EMBRAPA. Unidade de Execução de Pesquisa de Âmbito Estadual de Dourados. |
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Título: |
IAPAR 3-Aracatu: nova variedade de trigo recomendada para Mato Grosso do Sul. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Dourados, 1982. |
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Páginas: |
1 p. |
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Série: |
(EMBRAPA-UEPAE Dourados. Noticiário, 50). |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Cultivar IAPAR 3 Aracatu; Mato Grosso do Sul. |
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Thesagro: |
Cerrado; Trigo; Triticum Aestivum. |
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Thesaurus NAL: |
Brazil; varieties; wheat. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/73665/1/FOL-2344.pdf
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Marc: |
LEADER 00669nam a2200229 a 4500
001 1231146
005 2013-01-10
008 1982 bl uuuu u0uu1 u #d
100 1 $aEMBRAPA. Unidade de Execução de Pesquisa de Âmbito Estadual de Dourados.
245 $aIAPAR 3-Aracatu$bnova variedade de trigo recomendada para Mato Grosso do Sul.
260 $aDourados$c1982
300 $a1 p.
490 $a(EMBRAPA-UEPAE Dourados. Noticiário, 50).
650 $aBrazil
650 $avarieties
650 $awheat
650 $aCerrado
650 $aTrigo
650 $aTriticum Aestivum
653 $aBrasil
653 $aCultivar IAPAR 3 Aracatu
653 $aMato Grosso do Sul
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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